Purpose
We have recently reported that application of carbodiimide-derivatized hyaluronic
acid and gelatin (cd-HA gelatin) to a peroneus longus tendon graft increased tendon
graft gliding ability and decreased work of flexion compared with untreated grafts
in a canine model in vivo. In this study, we investigated the effect of this modification on adhesions, stiffness,
strength of the distal attachment, and fibroblast count.
Methods
A total of 24 dogs were used for this study. The peroneus longus tendons of each hind
leg were grafted into the 2nd and 5th digits of one forepaw in each dog. One peroneus
longus tendon was treated with cd-HA gelatin prior to grafting, and the other one
was immersed in 0.9% saline solution as a control. Animals were killed 1, 3, or 6
weeks postoperatively.
Results
The adhesion score of cd-HA gelatin–treated tendons was significantly less than that
in the saline-treated tendons at all time points. There was no significant difference
in the indentation stiffness between HA- and saline-treated grafts at any time point.
For the ultimate force at the distal attachment, there was a significant difference
among the time points, with a steady increase over time, but no significant difference
between treated and control tendons at any time point. There was no significant difference
in fibroblast count between treated and control tendons at any time point.
Conclusions
Although gross adhesion formation was less, there was no significant difference in
strength at the distal tendon-bone interface, cellularity, or tendon graft stiffness
when comparing saline-treated and cd-HA gelatin–treated tendon grafts in vivo.
Key words
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Article info
Publication history
Accepted:
March 6,
2007
Received:
September 25,
2006
Footnotes
No benefits in any form have been received or will be received from a commercial party related directly or indirectly to the subject of this article.
Supported by grants from the Orthopaedic Research and Education Foundation and from NIH/NIAMS (AR49407).
Identification
Copyright
© 2007 American Society for Surgery of the Hand. Published by Elsevier Inc. All rights reserved.
